tailieunhanh - Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine. | Ann. For. Sci. 59 2002 669-673 INRA EDP Sciences 2002 DOI forest 2002054 669 Original article Structural and functional characterization of the 5 upstream region of a glutamine synthetase gene from Scots pine Concepción Avilaa Francisco R. Cantóna Pilar Barnesteina María-Fernanda Suáreza Pierre Marraccinia Manuel Reyb Jaime M. Humarac Ricardo Ordásc and Francisco M. Cánovasa a Departamento de Biología Molecular y Bioquímica Instituto Andaluz de Biotecnología Unidad Asociada UMA-CSIC Facultad de Ciencias Universidad de Malaga 29071 Malaga Spain b Laboratorio Fisiología y Biotecnología Vegetal Facultad de Ciencias Universidad de Vigo 36200 Vigo Spain cLaboratorio Fisiología Vegetal Departamento BOS Universidad de Oviedo 33071 Oviedo Spain Received 5 July 2001 accepted 25 January 2002 Abstract - We report here the isolation and characterization of a genomic clone encoding Scots pine P. sylvestris cytosolic glutamine synthetase GS1a. The clone contains the 5 half of the gene including part of the coding region organized in seven exons interrupted by 6 introns and 980 bp upstream of the translation initiation codon. Earlier experiments carried out in our lab have shown that the GS1a gene is expressed in a light dependent fashion during the initial stages of Scots pine development. These data suggest a specific role for GS1a in ammonia assimilation in photosynthetic tissues of pine seedlings similar to the physiological role of GS2 in angiosperms. We have used a transcriptional fusion to uidA to transform pine cotyledons and Arabidopsis and demonstrated the ability of this 5 -upstream sequence to drive gene expression in both species and light regulation in Arabidopsis. cytosolic glutamine synthetase conifer gene expression N metabolism Résumé - Caractérisation structurale et fonctionnelle de la region 5 du gène de la glutamine synthetase du pin sylvestre. Un clone géno-mique codant la glutamine synthetase cytosolique GS1a de pin sylvestre P. sylvestris a été .