tailieunhanh - Báo cáo khoa học: "A high density genetic map of maritime pine based on AFLPs"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: A high density genetic map of maritime pine based on AFLPs. | Ann. For. Sci. 59 2002 627-636 INRA EDP Sciences 2002 DOI forest 2002048 627 Original article A high density genetic map of maritime pine based on AFLPs I o X 71 4 o ITĨÌ a 1 1 T A A o Ì11 f a QJ o Ý1 0 h x 11 tY Ofb To TÌ A A ct T f I IT nẤrì z a David Chagné Céline Lalanne Delphine Madur SatiSh Kumar Jean-Marc Frigério I o tKori no X T 1 f -r a X tonri o Tì ÍO lla A T Ti z l 1 I X o A ĩí 1 lt oc A A o ÍT o X5 z l 1 _ I A o T M i T _X m OffO Ýc Catherine Krier Stéphane Decroocq Arnould Savouré Magida Bou-Dagher-Kharrat Evangelista Bertocchia Jean Bracha and Christophe Plomiona aINRA Équipe de Génétique et Amelioration des Arbres Forestiers 69 route d Arcachon 33612 Cestas Cedex France b Forest Research Applications of Genomic Science Sala Street Rotorua 3021 New Zealand cPhysiologie Cellulaire et Moléculaire des Plantes UMR 7632 CNRS Université Pierre et Marie Curie case 156 4 Place Jussieu 75252 Paris Cedex 05 France Received 16 August 2001 accepted 13 February 2002 Abstract - We constructed a high-density linkage map of maritime pine Pinus pinaster Ait. based on AFLP Amplified Fragment Length Polymorphism markers using a three-generation outbred pedigree. In a first step male and female maps were established independently with test-cross markers segregating 1 1 presence absence of the amplified fragment in the full-sib progeny . In a second step both maps were merged using intercross markers segregating 3 1 in the progeny. A combination of MAPMAKER and JOINMAP softwares was used for the mapping process. A consensus map was obtained and is available at URL http genetics pinus Map3 . It covers 1441 cM and comprises a total of 620 AFLP markers on 12 linkage groups. The physical size of the maritime pine genome pg 2C was measured by flow cytometry providing a physical genetic size ratio of Mb cM. This map will be used to dissect the genetic architecture of economically growth wood quality and ecologically water-use