tailieunhanh - Báo cáo y học: " Neuroprotective peptide ADNF-9 in fetal brain of C57BL/6 mice exposed prenatally to alcohol"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Neuroprotective peptide ADNF-9 in fetal brain of C57BL/6 mice exposed prenatally to alcohol. | Sari et al. Journal of Biomedical Science 2011 18 77 http content 18 1 77 BIN sc The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Neuroprotective peptide ADNF-9 in fetal brain of C57BL 6 mice exposed prenatally to alcohol r - 1 2 3 2 4 Youssef Sari Zaneer M Segu Ahmed YoussefAgha Jonathan A Karty and Dragan Isailovic Abstract Background A derived peptide from activity-dependent neurotrophic factor ADNF-9 has been shown to be neuroprotective in the fetal alcohol exposure model. We investigated the neuroprotective effects of ADNF-9 against alcohol-induced apoptosis using TUNEL staining. We further characterize in this study the proteomic architecture underlying the role of ADNF-9 against ethanol teratogenesis during early fetal brain development using liquid chromatography in conjunction with tandem mass spectrometry LC-MS MS . Methods Pregnant C57BL 6 mice were exposed from embryonic days 7-13 E7-E13 to a 25 ethanol-derived calorie 25 EDC Alcohol ALC diet a 25 EDC diet simultaneously administered . ADNF-9 ALC ADNF-9 or a pair-fed PF liquid diet. At E13 fetal brains were collected from 5 dams from each group weighed and frozen for LC-MS MS procedure. Other fetal brains were fixed for TUNEL staining. Results Administration of ADNF-9 prevented alcohol-induced reduction in fetal brain weight and alcohol-induced increases in cell death. Moreover individual fetal brains were analyzed by LC-MS MS. Statistical differences in the amounts of proteins between the ALC and ALC ADNF-9 groups resulted in a distinct data-clustering. Significant upregulation of several important proteins involved in brain development were found in the ALC ADNF-9 group as compared to the ALC group. Conclusion These findings provide information on potential mechanisms underlying the neuroprotective effects of ADNF-9 in the fetal alcohol exposure model. Background Fetal .