tailieunhanh - Báo cáo y học: "Pu-Erh tea and GABA attenuates oxidative stress in kainic acid-induced status epilepticu"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Pu-Erh tea and GABA attenuates oxidative stress in kainic acid-induced status epilepticus. | Hou Journal of Biomedical Science 2011 18 75 http content 18 1 75 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Pu-Erh tea and GABA attenuates oxidative stress in kainic acid-induced status epilepticus Chien-Wei Hou Abstract Background Pu-Erh tea is one of the most-consumed beverages due to its taste and the anti-anxiety-producing effect of the gamma-aminobutyric acid GABA if contains. However the protective effects of Pu-Erh tea and its constituent GABA to kainic acid KA -induced seizure have not been fully investigated. Methods We analyzed the effect of Pu-Erh tea leaf PETL and GABA on KA-induced neuronal injury in vivo and in vitro. Results PETL and GABA reduced the maximal seizure classes predominant behavioral seizure patterns and lipid peroxidation in male FVB mice with status epilepticus. PETL extracts and GABA were effective in protecting KA-treated PC12 cells in a dose-dependent manner and they decreased Ca2 release ROS production and lipid peroxidation from KA-stressed PC12 cells. Western blot results revealed that mitogen-activated protein kinases MAPKs RhoA and cyclo-oxygenase-2 COX-2 expression were increased in PC12 cells under KA stress and PETL and GABA significantly reduced COX-2 and p38 MAPK expression but not that of RhoA. Furthermore PETL and GABA reduced PGE2 production from KA-induced PC12 cells. Conclusions Taken together PETL and GABA have neuroprotective effects against excitotoxins that may have clinical applications in epilepsy. Keywords GABA Epilepticus MAPKs ROS COX-2 Background Pu-erh tea is one of the most widely consumed beverages in the Orient. In recent years studies the possible investigating health benefits of Pu-erh tea have shown salutary effects on oxidative stress cancer cholesterol levels blood pressure and blood sugar and the bacterial flora of the intestines 1-6 . Soluble ingredients in .