tailieunhanh - Báo cáo y học: "Slow conduction and gap junction remodeling in murine ventricle after chronic alcohol ingestion"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Slow conduction and gap junction remodeling in murine ventricle after chronic alcohol ingestion. | Lai et al. Journal of Biomedical Science 2011 18 72 http content 18 1 72 BIN sc The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Slow conduction and gap junction remodeling in murine ventricle after chronic alcohol ingestion 1 1 1 12 3 1 Yu-Jun Lai Chung-Lieh Hung Ray-Ching Hong Ya-Ming Tseng Cheng-I Lin Yu-Shien Ko Cheng-Ho Tsai and Hung-I Yeh1 Abstract Background Long-term heavy alcohol drinkers are prone to the development of cardiac arrhythmia. To understand the mechanisms we evaluated the cardiac structural and electrophysiological changes in mice chronically drinking excessive alcohol. Results Male C57BL 6J mice were given 36 alcohol in the drinking water. Those given blank water were used as control. Twelve weeks later the phenotypic characteristics of the heart including gap junctions and electrical properties were examined. In the alcohol group the ventricles contained a smaller size of cardiomyocytes and a higher density of capillary networks compared to the control. Western blots showed that after drinking alcohol the content of connexin43 Cx43 protein in the left ventricle was increased by 18 p . Consistently immunoconfocal microscopy demonstrated that Cx43 gap junctions were up-regulated in the alcohol group with a disorganized distribution compared to the control. Optical mapping showed that the alcohol group had a reduced conduction velocity 40 18 vs 60 7 cm sec p and a higher incidence of ventricular tachyarrhythmia 62 vs 30 p . Conclusion Long-term excessive alcohol intake resulted in extensive cardiac remodeling including changes in expression and distribution of gap junctions growth of capillary network reduction of cardiomyocyte size and decrease of myocardial conduction. Keywords alcohol arrhythmia remodeling gap junctions optical mapping Background Excessive alcohol ingestion is harmful to the heart 1-4 .

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