tailieunhanh - Báo cáo y học: " Differential toll-like receptor 3 (TLR3) expression and apoptotic response to TLR3 agonist in human neuroblastoma cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Differential toll-like receptor 3 (TLR3) expression and apoptotic response to TLR3 agonist in human neuroblastoma cells. | Chuang et al. Journal of Biomedical Science 2011 18 65 http content 18 1 65 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Differential toll-like receptor 3 TLR3 expression and apoptotic response to TLR3 agonist in human neuroblastoma cells Jiin-Haur Chuang1 2 Hui-Ching Chuang2 3 Chao-Cheng Huang4 Chia-Ling Wu1 Yung-Ying Du1 Mei-Lang Kung5 Chih-Hao Chen6 San-Cher Chen7 and Ming-Hong Tai5 7 Abstract Background Toll-like receptor-3 TLR-3 is a critical component of innate immune system against dsRNA viruses and is expressed in the central nervous system. However it remains unknown whether TLR3 may serve as a therapeutic target in human neuroblastoma NB . Methods TLR3 expression in human NB samples was examined by immunohistochemical analysis. Quantitative RT-PCR and western blot was used to determine TLR3 expression in three human NB cell lines. The effect of TLR3 agonist polyinosinic-polycytidylic acid poly I C on the growth of human NB cells was evaluated by WST-1 cell proliferation assay flow cytometry analysis and immunoblot analysis. Blockade of TLR3 signaling was achieved using TLR3 neutralizing antibody small interference RNA and 2-aminopurine 2-AP an inhibitor of protein kinase R PKR an interferon-induced double-stranded RNA-activated protein kinase. Results In immunohistochemical studies TLR3 mainly expressed in the cytoplasm of ganglion cells and in some neuroblastic cells but not in the stromal cells in human NB tissues. Among three human NB cell lines analyzed TLR3 was significantly up-regulated in SK-N-AS cells at mRNA and protein level compared with other two low TLR3- expressing NB cells. Treatment with poly I C elicited significant growth inhibition and apoptosis only in high TLR3-expressing SK-N-AS cells but not in low TLR3-expressing SK-N-FI and SK-N-DZ cells. Moreover poly I C treatment significantly stimulated the .