tailieunhanh - Báo cáo y học: "BMP-2 gene-fibronectin-apatite composite layer enhances bone formation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: BMP-2 gene-fibronectin-apatite composite layer enhances bone formation. | Zhang et al. Journal of Biomedical Science 2011 18 62 http content 18 1 62 JOURNAL OF BIOMEDICAL SCIENCE The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan RESEARCH Open Access BMP-2 gene-fibronectin-apatite composite layer enhances bone formation 14 1 3 w - 3 3 2 Wei Zhang 1 Hideo Tsurushima 1 Ayako Oyane Yushin Yazaki Yu Sogo Atsuo Ito and Akira Matsumura Abstract Background Safe and efficient gene transfer systems are needed for tissue engineering. We have developed an apatite composite layer including the bone morphogenetic protein-2 BMP-2 gene and fibronectin FB and we evaluated its ability to induce bone formation. Methods An apatite composite layer was evaluated to determine the efficiency of gene transfer to cells cultured on it. Cells were cultured on a composite layer including the BMP-2 gene and FB and BMP-2 gene expression BMP-2 protein concentrations alkaline phosphatase ALP activity and osteocalcin OC concentrations were measured. A bone defect on the cranium of rats was treated with hydroxyapatite HAP -coated ceramic buttons with the apatite composite layer including the BMP-2 gene and FB HAP-BMP-FB . The tissue concentration of BMP-2 bone formation and the expression levels of the BMP-2 ALP and OC genes were all quantified. Results The apatite composite layer provided more efficient gene transfer for the cultured cells than an apatite composite layer without FB. The BMP-2 concentration was approximately 100 600 pg mL in the cell-culture medium. Culturing the cells on the apatite composite layer for 27 days increased ALP activity and OC concentrations. In animal experiments the tissue concentrations of BMP-2 were over 100 pg mg in the HAP-BMP-FB group and approximately 50 pg mg in the control groups. Eight weeks later bone formation was more enhanced in the HAP-BMP-FB group than in the control groups. In the tissues surrounding the HAP button the gene expression .