tailieunhanh - Báo cáo y học: "JNK suppression is essential for 17b-Estradiol inhibits prostaglandin E2-Induced uPA and MMP9 expressions and cell migration in human LoVo colon cancer cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: JNK suppression is essential for 17b-Estradiol inhibits prostaglandin E2-Induced uPA and MMP9 expressions and cell migration in human LoVo colon cancer cells. | Hsu et al. Journal of Biomedical Science 2011 18 61 http content 18 1 61 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access JNK suppression is essential for 17p-Estradiol inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells Hsi-Hsien Hsu1 2 Wei-Syun Hu3 4 Yueh-Min Lin5 6 Wei-Wen Kuo7 Li-Mien Chen8 Wei-Kung Chen9 Jin-Ming Hwang10 Fuu-Jen Tsai9 Chung-Jung Liu12 133 and Chih-Yang Huang11 14 15 3 Abstract Background Epidemiological studies demonstrate that the incidence and mortality rates of colorectal cancer in women are lower than in men. However it is unknown if 17p-estradiol treatment is sufficient to inhibit prostaglandin E2 PGE2 -induced cellular motility in human colon cancer cells. Methods We analyzed the protein expression of urokinase plasminogen activator uPA tissue plasminogen activator tPA matrix metallopeptidases MMPs plasminogen activator inhibitor-1 PAI-1 and tissue inhibitor of metalloproteinases TIMPs and the cellular motility in PGE2-stimulated human LoVo cells. 17p-Estradiol and the inhibitors including LY294002 Akt activation inhibitor U0126 ERK1 2 inhibitor SB203580 p38 MAPK inhibitor SP600125 JNK1 2 inhibitor QNZ NFkB inhibitor and ICI 182 780 were further used to explore the inhibitory effects of 17p-estradiol on PGE2-induced LoVo cell motility. Student s t-test was used to analyze the difference between the two groups. Results Upregulation of urokinase plasminogen activator uPA tissue plasminogen activator tPA and matrix metallopeptidases MMPs is reported to associate with the development of cancer cell mobility metastasis and subsequent malignant tumor. After administration of inhibitors including LY294002 U0126 SB203580 SP600125 or QNZ we found that PGE2 treatment up-regulated uPA and MMP-9 expression via JNK1 2 signaling pathway thus promoting cellular .