tailieunhanh - Báo cáo y học: "Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway. | Liu et al. Journal of Biomedical Science 2011 18 58 http content 18 1 58 BIN sc The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway Shan-shan Liu1 Xiang-mei Chen3 Hong-xia Zheng1 Shu-liang Shi1 and Yu Li1 2 Abstract Background Rab GTPases function as modulators in intracellular transport. Rab5a a member of the Rab subfamily of small GTPases is an important regulator of vesicle traffic from the plasma membrane to early endosomes. Recent findings have reported that Rab5a gene was involved in the progression of cancer. In the present study we investigated the effect of Rab5a on cervical cancer invasion and metastasis and the molecular mechanism underlying the involvement of Rab5a. Methods Rab5a expression was assessed by immunohistochemical analysis on a cervical cancer tissue microarray. RNA interference RNAi was performed to knock down the endogenous expression of Rab5a gene in HeLa and SiHa cells. Cell motility was evaluated using invasion assay and wound migration assay in vitro. The expression levels of integrin-associated molecules were detected by Western blot and immunofluorescence. Results We found that Rab5a was expressed at a high level in cervical cancer tissues. Silencing of Rab5a expression significantly decreased cancer cell motility and invasiveness. The down-regulation of integrin-associated focal adhesion signaling molecules was further detected in Rab5a knockdown cells. Meanwhile active GTP-bound Rac1 Cdc42 and RhoA were also down-regulated accompanied with the reduction in the number and size of filopodia and lamellipodia. Conclusions Taken together these data suggest that Rab5a functions in regulating the invasion phenotype and we propose that this regulation may be via integrin-mediated signaling pathway in .