tailieunhanh - Báo cáo y học: "Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cell"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cells. | Chang et al. Journal of Biomedical Science 2011 18 54 http content 18 1 54 tì NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cells You-Kang Chang1 2 3 Ming-Hsiang Chen4 Yi-Hung Chiang1 5 Yu-Fan Chen4 Wei-Hsien Ma4 Chian-You Tseng4 Bin-Wen Soong6 7 Jennifer H Ho8 9 10 and Oscar K Lee1 4 11 Abstract Background Spinocerebellar ataxia SCA refers to a disease entity in which polyglutamine aggregates are overproduced in Purkinje cells PCs of the cerebellum as well as other neurons in the central nervous system and the formation of intracellular polyglutamine aggregates result in the loss of neurons as well as deterioration of motor functions. So far there is no effective neuroprotective treatment for this debilitating disease although numerous efforts have been made. Mesenchymal stem cells MSCs possess multi-lineage differentiation potentials as well as immuno-modulatory properties and are theoretically good candidates for SCA treatment. The purpose of this study is to investigate whether transplantation of human MSCs hMSCs can rescue cerebellar PCs and ameliorate motor function deterioration in SCA in a pre-clinical animal model. Method Transgenic mice bearing poly-glutamine mutation in ataxin-2 gene C57BL 6J SCA2 transgenic mice were serially transplanted with hMSCs intravenously or intracranially before and after the onset of motor function loss. Motor function of mice was evaluated by an accelerating protocol of rotarod test every 8 weeks. Immunohistochemical stain of whole brain sections was adopted to demonstrate the neuroprotective effect of hMSC transplantation on cerebellar PCs and engraftment of hMSCs into mice brain. Results Intravenous transplantation of hMSCs effectively improved rotarod .