tailieunhanh - Báo cáo y học: "Early innate immune events induced by prolonged cold ischemia exacerbate allograft vasculopathy"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Early innate immune events induced by prolonged cold ischemia exacerbate allograft vasculopathy. | Devitt et al. Journal of Cardiothoracic Surgery 2011 6 2 http content 6 1 2 JCTS JOURNAL OF CARDIOTHORACIC SURGERY RESEARCH ARTICLE Open Access Early innate immune events induced by prolonged cold ischemia exacerbate allograft vasculopathy Jennifer J Devitt1 Chelsey L King2 Timothy DG Lee1 2 3 Camille L Hancock Friesen1 2 Abstract Background Ischemia reperfusion induced innate immune injury is inescapable in solid organ transplantation. Prolonged cold ischemia exacerbates the primary manifestation of late graft rejection allograft vasculopathy AV . The relationship between prolonged cold ischemia and late graft events is unclear and the subject of this study. Methods Aortic interposition transplants were performed between fully disparate mice treated with CyclosporineA. Allografts were exposed to 20 min or 60 min of cold ischemia and harvested between 1 d-6 wk. Lesion size smooth muscle cells SMC neutrophils N0 and CD8 T cells were quantified. Results Early SMC loss was identical in both groups. When compared to 20 min cold ischemia grafts exposed to 60 min exhibited greater early N0 influx greater SMC proliferation but fewer medial SMC at 1 wk and 2 wk. Subsequently earlier and greater CD8 T cell infiltration were seen in the 60 min group with larger lesions at every time point. Conclusions These data suggest that the larger neointimal lesions in grafts exposed to 60 min cold ischemia result from enhanced early innate immune events resulting in impaired SMC recovery and subsequent increased adaptive immune response. Introduction Despite the introduction of mechanical assist devices to stabilize cardiac function cardiac transplantation remains the primary treatment for end stage heart failure. Unfortunately the long term 10 yr survival of cardiac transplants has not improved in the last three decades and still hovers at approximately 50 in most centres 1 . The primary manifestation of late graft rejection in cardiac transplants is .