tailieunhanh - Báo cáo y học: " Functional cooperation of of IL-1b and RGS4 in the brachial plexus avulsion mediated brain reorganizatio"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Functional cooperation of of IL-1b and RGS4 in the brachial plexus avulsion mediated brain reorganization. | Li et al. Journal of Brachial Plexus and Peripheral Nerve Injury 2010 5 18 http content 5 1 18 . JOURNAL OF BRACHIAL PLEXUS AND 11 PERIPHERAL NERVE INJURY RESEARCH ARTICLE Open Access Functional cooperation of of IL-1p and RGS4 in the brachial plexus avulsion mediated brain reorganization Jifeng Li Hui Zhao Pengbo Luo Yudong Gu Abstract Backgrounds There is considerable evidence that central nervous system is continuously modulated by activity behavior and skill acquisition. This study is to examine the reorganization in cortical and subcortical regions in response to brachial plexus avulsion. Methods Adult C57BL 6 mice were divided into four groups control 1 3 and 6 month of brachial plexus avulsion. IL-1p IL-6 and RGS4 expression in cortex brainstem and spinal cord were detected by BiostarM-140 s microarray and real-time PCR. RGS4 subcellular distribution and modulation were further analyzed by primary neuron culture and Western Blot. Results After 1 3 and 6 months of brachial plexus avulsion 49 0 up 49 down 29 17 up 12 down 13 9 up 4 down genes in cerebral cortex 40 8 up 32 down 11 7 up 4 down 137 63 up 74 down in brainstem 27 14 up 13 down 33 18 up 15 down 60 29 up 31 down in spinal cord were identified. Among the regulated gene IL-1p and IL-6 were sustainable enhanced in brain stem while PKACp and RGS4 were up-regulated throughout cerebral cortex brainstem and spinal cord in 3 and 6 month of nerve injury. Intriguingly subcellular distribution of RGS4 in above three regions was dependent on the functional correlation of PKA and IL-1p. Conclusion Data herein indicated that brachial plexus avulsion could efficiently initiate and perpetuate the brain reorganization. Network involved IL-1p and RGS4 signaling might implicate in the re-establish and strengthening of the local circuits at the cortical and subcortical levels. Backgrounds Neuroplasticity is the changing of neurons and the organization of their networks which may happen through adding new