tailieunhanh - Báo cáo y học: "Storage and allogeneic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model'

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Storage and allogeneic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model. | Nakayama et al. Journal of Brachial Plexus and Peripheral Nerve Injury 2010 5 17 http content 5 1 17 JOURNAL OF BRACHIAL PLEXUS AND PERIPHERAL NERVE INJURY RESEARCH ARTICLE Open Access Storage and allogeneic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model 1 4 5 2 2 Ken Nakayama Ryosuke Kakinoki Ryosuke Ikeguchi Tomoyuki Yamakawa Soichi Ohta Satoshi Fujita Takashi Noguchi 2 Scott FM Duncan6 Suong-Hyu Hyon7 Takashi Nakamura2 3 Abstract Background In our previous study allogeneic-transplanted peripheral nerve segments preserved for one month in a polyphenol solution at 4 C could regenerate nerves in rodents demonstrated the same extent of nerve regeneration as isogeneic fresh nerve grafts. The present study investigated whether the same results could be obtained in a canine model. Methods A sciatic nerve was harvested from a male beagle dog divided into fascicules of mm diameter and stored in a polyphenol solution 1 mg ml for one month at 4 C. The nerve fascicles were transplanted into 10 female beagle dogs to bridge 3-cm right ulnar nerve gaps. In the left ulnar nerve in each dog a 3-cm nerve segment was harvested turned in the opposite direction and sutured in situ. Starting one day before transplantation the immunosuppressant FK506 was administered subcutaneously at doses of mg kg daily in four dogs group mg kg daily in four dogs group or mg kg every other day in two dogs group . Twelve weeks after surgery electrophysiological and morphological studies were performed to assess the regeneration of the right and left ulnar nerves. The data for the right ulnar nerve were expressed as percentages relative to the left ulnar nerve. Polymerase chain reaction PCR was used to identify the sexdetermining region of the Y-chromosome Sry and p-actin to investigate whether cells of donor origin remained in the allogeneic nerve segments. FK506 concentration was measured in blood