tailieunhanh - Báo cáo y học: "Differential cellular FGF-2 upregulation in the rat facial nucleus following axotomy, functional electrical stimulation and corticosterone: a possible therapeutic target to Bell’s pals"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Differential cellular FGF-2 upregulation in the rat facial nucleus following axotomy, functional electrical stimulation and corticosterone: a possible therapeutic target to Bell’s palsy. | Coracini et al. Journal of Brachial Plexus and Peripheral Nerve Injury 2010 5 16 http content 5 1 16 JOURNAL OF BRACHIAL PLEXUS AND PERIPHERAL NERVE INJURY RESEARCH ARTICLE Open Access Differential cellular FGF-2 upregulation in the rat facial nucleus following axotomy functional electrical stimulation and corticosterone a possible therapeutic target to Bell s palsy Karen F Coracini Caio J Fernandes Almir F Barbarini César M Silva Rodrigo T Scabello Gabriela P Oliveira Gerson Chadi Abstract Background The etiology of Bell s palsy can vary but anterograde axonal degeneration may delay spontaneous functional recovery leading the necessity of therapeutic interventions. Corticotherapy and or complementary rehabilitation interventions have been employed. Thus the natural history of the disease reports to a neurotrophic resistance of adult facial motoneurons leading a favorable evolution however the related molecular mechanisms that might be therapeutically addressed in the resistant cases are not known. Fibroblast growth factor-2 FGF-2 pathway signaling is a potential candidate for therapeutic development because its role on wound repair and autocrine paracrine trophic mechanisms in the lesioned nervous system. Methods Adult rats received unilateral facial nerve crush transection with amputation of nerve branches or sham operation. Other group of unlesioned rats received a daily functional electrical stimulation in the levator labii superioris muscle 1 mA 30 Hz square wave or systemic corticosterone 10 mgkg-1 . Animals were sacrificed seven days later. Results Crush and transection lesions promoted no changes in the number of neurons but increased the neurofilament in the neuronal neuropil of axotomized facial nuclei. Axotomy also elevated the number of GFAP astrocytes 143 after crush 277 after transection and nuclear FGF-2 57 after transection in astrocytes confirmed by two-color immunoperoxidase in the ipsilateral facial nucleus. Image analysis reveled