tailieunhanh - Báo cáo y học: "valuation of endoscopic vein extraction on structural and functional viability of saphenous vein endothelium"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Evaluation of endoscopic vein extraction on structural and functional viability of saphenous vein endothelium. | Hussaini et al. Journal of Cardiothoracic Surgery 2011 6 82 http content 6 1 82 JCTS JOURNAL OF CARDIOTHORACIC SURGERY RESEARCH ARTICLE Open Access Evaluation of endoscopic vein extraction on structural and functional viability of saphenous vein endothelium Bader E Hussaini1 2 3 Xiu-Gui Lu1 2 3 J Alan Wolfe4 and Hemant S Thatte1 2 3 Abstract Objectives Endothelial injury during harvest influences graft patency post CABG. We have previously shown that endoscopic harvest causes structural and functional damage to the saphenous vein SV endothelium. However causes of such injury may depend on the extraction technique. In order to assess this supposition we evaluated the effect of VirtuoSaph endoscopic SV harvesting technique VsEVH on structural and functional viability of SV endothelium using multiphoton imaging biochemical and immunofluorescence assays. Methods Nineteen patients scheduled for CABG were prospectively identified. Each underwent VsEVH for one portion and No-touch open SV harvesting OSVH for another portion of the SV. A two cm segment from each portion was immersed in GALA conduit preservation solution and transported overnight to our lab for processing. The segments were labeled with fluorescent markers to quantify cell viability calcium mobilization and generation of nitric oxide. Morphology expression localization and stability of endothelial caveolin eNOS von Willebrand factor and cadherin were evaluated using immunofluorescence Western blot and multiphoton microscopy MPM . Results Morphological biochemical and immunofluorescence parameters of viability structure and function were well preserved in VsEVH group as in OSVH group. However tonic eNOS activity agonist-dependent calcium mobilization and nitric oxide production were partially attenuated in the VsEVH group. Conclusions This study indicates that VirtuoSaph endoscopic SV harvesting technique preserves the structural and functional viability of SV endothelium but .