tailieunhanh - Báo cáo y học: "Leukotriene biosynthesis inhibition ameliorates acute lung injury following hemorrhagic shock in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Leukotriene biosynthesis inhibition ameliorates acute lung injury following hemorrhagic shock in rats. | Al-Amran et al. Journal of Cardiothoracic Surgery 2011 6 81 http content 6 1 81 JCTS JOURNAL OF CARDIOTHORACIC SURGERY RESEARCH ARTICLE Open Access Leukotriene biosynthesis inhibition ameliorates acute lung injury following hemorrhagic shock in rats Fadhil G Al-Amran1 Najah R Hadi2 and Ali M Hashim2 Abstract Background Hemorrhagic shock followed by resuscitation is conceived as an insult frequently induces a systemic inflammatory response syndrome and oxidative stress that results in multiple-organ dysfunction syndrome including acute lung injury. MK-886 is a leukotriene biosynthesis inhibitor exerts an anti inflammatory and antioxidant activity. Objectives The objective of present study was to assess the possible protective effect of MK-886 against hemorrhagic shock-induced acute lung injury via interfering with inflammatory and oxidative pathways. Materials and methods Eighteen adult Albino rats were assigned to three groups each containing six rats group I sham group rats underwent all surgical instrumentation but neither hemorrhagic shock nor resuscitation was done group II Rats underwent hemorrhagic shock HS for 1 hr then resuscitated with Ringer s lactate 1 hr induced untreated group HS group III HS MK-886 mg kg . injection 30 min before the induction of HS and the same dose was repeated just before reperfusion period . At the end of experiment 2 hr after completion of resuscitation blood samples were collected for measurement of serum tumor necrosis factor-a TNF-a and interleukin-6 IL-6 . The trachea was then isolated and bronchoalveolar lavage fluid BALF was carried out for measurement of leukotriene B4 LTB4 leukotriene C4 LTC4 and total protein. The lungs were harvested excised and the left lung was homogenized for measurement of malondialdehyde MDA and reduced glutathione GSH and the right lung was fixed in 10 formalin for histological examination. Results MK-886 treatment significantly reduced the total lung injury .