tailieunhanh - Báo cáo y học: "The challenge to verify ceramide’s role of apoptosis induction in human cardiomyocytes a pilot study"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: The challenge to verify ceramide’s role of apoptosis induction in human cardiomyocytes a pilot study. | Usta et al. Journal of Cardiothoracic Surgery 2011 6 38 http content 6 1 38 JCTS JOURNAL OF CARDIOTHORACIC SURGERY RESEARCH ARTICLE Open Access The challenge to verify ceramide s role of apoptosis induction in human cardiomyocytes -a pilot study 1 2 3 2 2 4 Engin Usta Migdat Mustafi Ferruh Artunc Tobias Walker Vladimir Voth Hermann Aebert and Gerhard Ziemer1 Abstract Background Cardioplegia and reperfusion of the myocardium may be associated with cardiomyocyte apoptosis and subsequent myocardial injury. In order to establish a pharmacological strategy for the prevention of these events this study aimed to verify the reliability of our human cardiac model and to evaluate the pro-apoptotic properties of the sphingolipid second messenger ceramide and the anti-apoptotic properties of the acid sphingomyelinase inhibitor amitryptiline during simulated cardioplegia and reperfusion ex vivo. Methods Cardiac biopsies were retrieved from the right auricle of patients undergoing elective CABG before induction of cardiopulmonary bypass. Biopsies were exposed to ex vivo conditions of varying periods of cp rep 30 10 60 20 120 40 min . Groups I untreated control n 10 II treated control cp rep n 10 III cp rep ceramide n 10 IV cp rep amitryptiline n 10 and V cp rep ceramide amitryptiline n 10 . For detection of apoptosis anti-activated-caspase-3 and PARP-1 cleavage immunostaining were employed. Results In group I the percentage of apoptotic cardiomyocytes was significantly p low if compared to group II revealing a time-dependent increase. In group III ceramid increased and in group IV amitryptiline inhibited apoptosis significantly p . In contrast in group V under the influence of ceramide and amitryptiline the induction of apoptosis was partially suppressed. Conclusion Ceramid induces and amitryptiline suppresses apoptosis significantly in our ex vivo setting. This finding warrants further studies aiming to evaluate potential beneficial .

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