tailieunhanh - Báo cáo y học: " Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10."

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. | Soltermann et al. Journal of Clinical Bioinformatics 2011 1 25 http content 1 1 25 JOURNAL OF CLINICAL BIOINFORMATICS RESEARCH Open Access Automated ERCC1 immunochemistry on hybrid cytology tissue microarray of malignant effusions evaluation of antibodies 8F1 and D-10 Alex Soltermann Sandra Kilgus-Hawelski Silvia Behnke Martina Storz Holger Moch and Beata Bode Abstract Background The excision repair cross-complementation group 1 ERCC1 protein is the key enzyme of the nucleotide excision repair NER pathway. Loss of protein expression on immunohistochemistry is predictive for platinum-based chemotherapy response. Frequently the diagnosis of malignancy is made on cytologic effusion samples. Therefore we evaluated the staining quality of monoclonal anti-ERCC1 antibodies 8F1 and D-10 on microarrays of malignant pleural and peritoneal effusions by automated immunochemistry. Methods Cores from effusion cell blocks of 117 patients with 40 malignant cell clusters per whole section pleural n 75 peritoneal n 42 were assembled together with 30 histologic control cores from large tissue blocks lung breast and ovarian carcinoma each n 10 on hybrid cytology-tissue microarrays C TMA . Four immunochemistry protocols Mab 8F1 and D-10 CC1-mono Ventana and H2-60 Bond automat were performed. Immunoreactivity was semi-quantitatively scored for intensity and intensity multiplied by percentage staining H-score . Results Tumors were classified into female genital tract carcinoma n 39 lung adenocarcinoma n 23 mesothelioma n 15 unknown primary n 14 breast carcinoma n 10 gastro-intestinal carcinoma n 12 and other n 4 . On both platforms reproducible nuclear ERCC1 immunoreactivity was achieved with both antibodies although D-10 was slightly weaker and presented more background staining as well as more variation in the low expression range. No significant differences were found between cytologic and histologic cores. Using the 8F1 CC1-mono protocol lung and breast .