tailieunhanh - Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study. | Nomura et al. Journal of Clinical Bioinformatics 2011 1 23 http content 1 1 23 JOURNAL OF CLINICAL BIOINFORMATICS RESEARCH Open Access Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study h I S I S S r I I l I fs m I I 1 2 I I r x Cl II I 3 Xl z-i s Cl I I II4 I - S r I IX-s t A ỉ s m I irn5 I I I v m s I r I 6 It ỉ IXI p. s u- s 7 Masaharu Nomura Tetsuya rUKuaa Kiyonaga Fujii laKesni Kawamura Hiromasa iQjQ iviaKoto Kihara 3 8 12 2 1 YasuhiKo Bando Adi F Gazdar Masahiro Tsuboi Hisashi Oshiro ToshitaKa Nagao Tatsuo Ohira NorihiKo IKeda1 NoriKo Gotoh9 Harubumi Kato10 Gyorgy MarKo-Varga11 and Toshihide Nishimura1 3 7 Abstract Background Large cell neuroendocrine carcinoma LCNEC of the lung a subtype of large cell carcinoma LCC is characterized by neuroendocrine differentiation that small cell lung carcinoma SCLC shares. Pre-therapeutic histological distinction between LCNEC and SCLC has so far been problematic leading to adverse clinical outcome. We started a project establishing protein targets characteristic of LCNEC with a proteomic method using formalin fixed paraffin-embedded FFPE tissues which will help maKe diagnosis convincing. Methods Cancer cells were collected by laser microdissection from cancer foci in FFPE tissues of LCNEC n 4 SCLC n 5 and LCC n 5 with definite histological diagnosis. Proteins were extracted from the harvested sections trypsin-digested and subjected to HPLC mass spectrometry. Proteins identified by database search were semi-quantified by spectral counting and statistically sorted by pair-wise G-statistics. The results were immunohistochemically verified using a total of 10 cases for each group to confirm proteomic results. Results A total of 1981 proteins identified from the three cancer groups were subjected to pair-wise G-test under p and specificity of a protein s expression to LCNEC was checKed using a 3D plot .