tailieunhanh - báo cáo hóa học: " Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses | Wisnewski et al. Journal of Occupational Medicine and Toxicology 2011 6 6 http content 6 1 6 RESEARCH JOURNAL OF OCCUPATIONAL MEDICINE AND TOXICOLOGY Open Access Immune sensitization to methylene diphenyl diisocyanate MDI resulting from skin exposure albumin as a carrier protein connecting skin exposure to subsequent respiratory responses 1 2 21 1 2 Adam V Wisnewski Lan Xu Eve Robinson Jian Liu Carrie A Redlich Christina A Herrick Abstract Background Methylene diphenyl diisocyanate MDI a reactive chemical used for commercial polyurethane production is a well-recognized cause of occupational asthma. The major focus of disease prevention efforts to date has been respiratory tract exposure however skin exposure may also be an important route for inducing immune sensitization which may promote subsequent airway inflammatory responses. We developed a murine model to investigate pathogenic mechanisms by which MDI skin exposure might promote subsequent immune responses including respiratory tract inflammation. Methods Mice exposed via the skin to varying doses w v of MDI diluted in acetone olive oil were subsequently evaluated for MDI immune sensitization. Serum levels of MDI-specific IgG and IgE were measured by enzyme-linked immunosorbant assay ELISA while respiratory tract inflammation induced by intranasal delivery of MDI-mouse albumin conjugates was evaluated based on bronchoalveolar lavage BAL . Autologous serum IgG from skin only exposed mice was used to detect and guide the purification identification of skin proteins antigenically modified by MDI exposure in vivo. Results Skin exposure to MDI resulted in specific antibody production and promoted subsequent respiratory tract inflammation in animals challenged intranasally with MDI-mouse albumin conjugates. The degree of secondary respiratory tract inflammation and eosinophilia depended upon the primary skin exposure dose and was maximal in mice exposed to 1 MDI but paradoxically limited in