tailieunhanh - Báo cáo y học: "Mating rhythms of Drosophila: rescue of tim01 mutants by D. ananassae timeless'

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Mating rhythms of Drosophila: rescue of tim01 mutants by D. ananassae timeless. | BioMed Central Journal of Circadian Rhythms Research Open Access Mating rhythms of Drosophila rescue of tim01 mutants by D. ananassae timeless Izumi Nishinokubi1 Masami Shimoda2 and Norio Ishida 1 3 Address 1Clock Cell Biology National Institute of Advanced Industrial Science and Technology Institute of Molecular and Cell Biology Tsukuba 305-8566 Japan 2National Institute of Agrobiological Sciences Tsukuba 305-8634 Japan and institute of Applied Biochemistry University of Tsukuba Tsukuba 305-8576 Japan Email IzumiNishinokubi - i-nishinokubi@ Masami Shimoda - shimoda1@ Norio Ishida - Corresponding author Published 08 March 2006 Received 15 December 2005 Accepted 08 March 2006 Journal of Circadian Rhythms2006 4 4 doi 1740-3391-4-4 This article is available from http content 4 1 4 2006Nishinokubi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background It is reported that the circadian rhythms of female mating activity differ among Drosophila species and are controlled by an endogenous circadian clock. Here we found that the mating rhythm of D. ananassae differed from that of D. melanogaster. Moreover to evaluate the effect of clock gene products on mating activities we examined the mating activity of D. melanogaster timeless tim01 transgenic fly harboring heat-shock promotor driven-D. ananassae timeless tim gene hs-AT tim01 . Methods Flies were maintained under light dark LD cycles for several days and then they were transferred to constant dark DD conditions at 25 C. Transformant flies were heat-shocked for 30 min PZT or pZt PZT means Projected Zeitgeber Time at 37 C every day. Daily expressions of D. ananassae .