tailieunhanh - báo cáo hóa học: " Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways | Lee et al. Journal of Occupational Medicine and Toxicology 2010 5 17 http content 5 1 17 JOURNAL OF OCCUPATIONAL MEDICINE ANDTOXICOLOGY RESEARCH Open Access Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways Chung-Hsun Lee1 2 I-Hui Chen2 Chia-Rong Lee2 Chih-Hsien Chi1 Ming-CheTsai1 Jin-Lian Tsai2 and Hsiu-Fen Lin 2 3 4 Abstract Background Organotin compounds OTCs have been widely used as stabilizers in the production of plastic agricultural pesticides antifoulant plaints and wood preservation. The toxicity of triphenyltin TPT compounds was known for their embryotoxic neurotoxic genotoxic and immunotoxic effects in mammals. The carcinogenicity of TPT was not well understood and few studies had discussed the effects of OTCs on gap junctional intercellular communication GJIC of cells. Method In the present study the effects of triphenyltin chloride TPTC on GJIC in WB-F344 rat liver epithelial cells were evaluated using the scrape-loading dye transfer technique. Results TPTC inhibited GJIC after a 30-min exposure in a concentration- and time-dependent manner. Pre-incubation of cells with the protein kinase C PKC inhibitor did not modify the response but the specific MEK 1 inhibitor PD98059 and PI3K inhibitor LY294002 decreased substantially the inhibition of GJIC by TPTC. After WB-F344 cells were exposed to TPTC phosphorylation of Cx43 increased as seen in Western blot analysis. Conclusions These results show that TPTC inhibits GJIC in WB-F344 rat liver epithelial cells by altering the Cx43 protein expression through both MAPK and PI3-kinase pathways. Background Organotin compounds have been widely used as agricultural biocides antifouling agents in boat paint wood preservatives and stabilizers for polyvinylchloride polymers PVC in industry 1 2 . Triphenyltin TPT is an organotin compound which is widely used as fungicides on major food and .