tailieunhanh - Báo cáo y học: ""Time sweet time": circadian characterization of galectin-1 null mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: "Time sweet time": circadian characterization of galectin-1 null mice. | Casiraghi et al. Journal of Circadian Rhythms 2010 8 4 http content 8 1 4 JOURNAL OF CIRCADIAN RHYTHMS RESEARCH Open Access Time sweet time circadian characterization of galectin-1 null mice Leandro P Casiraghi 1 Diego O Croci2 Francoise Poirier3 Gabriel A Rabinovich2 and Diego A Golombek 1 Abstract Background Recent evidence suggests a two-way interaction between the immune and circadian systems. Circadian control of immune factors as well as the effect of immunological variables on circadian rhythms might be key elements in both physiological and pathological responses to the environment. Among these relevant factors galectin-1 is a member of a family of evolutionarily-conserved glycan-binding proteins with both extracellular and intracellular effects playing important roles in immune cell processes and inflammatory responses. Many of these actions have been studied through the use of mice with a null mutation in the galectin-1 Lgalsl gene. To further analyze the role of endogenous galectin-1 in vivo we aimed to characterize the circadian behavior of galectin-1 null Lgalsh h mice. Methods We analyzed wheel-running activity in light-dark conditions constant darkness phase responses to light pulses LP at circadian time 15 and reentrainment to 6 hour shifts in light-dark schedule in wild-type WT and Lgalsl - mice. Results We found significant differences in free-running period which was longer in mutant than in WT mice vs h p phase delays in response to LP vs circadian h p and also in alpha vs. circadian h p . Conclusions Given the effect of a null mutation on circadian period and entrainment we indicate that galectin-1 could be involved in the regulation of murine circadian rhythmicity. This is the first study implicating galectin-1 in the mammalian circadian system. Background Circadian systems represent an endogenous mechanism adapted to cycling environmental conditions. In mammals the .