tailieunhanh - báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry | Journal of Occupational Medicine and Toxicology Methodology BioMed Central Open Access Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry Luis M Gonzalez-Reche1 Anita K Musiol1 Alice Muller-Lux1 Thomas Kraus 1 and Thomas Goen1 2 Address 1Institute and Outpatient-Clinic for Occupational and Social Medicine University Hospital Aachen University of Technology Pauwelsstrasse 30 D-52074 Aachen Germany and 2Institute for Occupational Social and Environmental Medicine University Erlangen-Nuremberg Schillerstr. 29 D-91054 Erlangen Germany Email Luis M Gonzalez-Reche - arbeitsmedizin@ Anita K Musiol - amusiol@ Alice Muller- Lux - Thomas Kraus - tkraus@ Thomas Goen - Corresponding author Published 17 May 2006 Received 20 January 2006 Journal of Occupational Medicine and Toxicology 2006 1 5 doi 1745-6673-1-5 Accepted 17 May 2006 This article is available from http content 1 1 5 2006 Gonzalez-Reche et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Determinations of inflammatory markers in exhaled breath condensate were used to assess airway inflammation. The most applied method for this kind of determination is enzyme immunoassay. For research purposes to find new or to relate concrete biomarkers to different pulmonary diseases a simultaneous determination of different inflammatory markers would be advantageous. Methods We developed an analytical method with on-line clean up and enrichment steps to determine 12 different inflammatory .