tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 59

Handbook of Microbiological Media, Fourth Edition part 59 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Desulfuromonas acetexigenes Medium 575 Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to . Mix thoroughly. Sparge with 80 N2 20 CO2. Filter sterilize. Na-pyruvate Solution Composition per Preparation of Na-pyruvate Solution Add Na-pyruvate to dis-tilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Filter sterilize. Seven Vitamin Solution Composition per liter Pyridoxine Nicotinic Vitamin Calcium p-Aminobenzoic D Preparation of Seven Vitamin Solution Add components to dis-tilled deionized water and bring volume to . Sparge with 100 N2. Mix thoroughly. Filter sterilize. Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Sulfur is sterilized by steaming for 3 hr on each of 3 successive days. Prepare and dispense medium under 80 N2 20 CO2 gas atmosphere. Add components except sulfur NaHCO3 solution Na-pyruvate solution Na2S-9H2O solution seven vitamin solution and trace elements solution SL-10 to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically and anaerobically add sterile sulfur NaHCO3 solution Na-pyruvate solution Na2S-9H2O solution seven vitamin solution and trace elements solution SL-10. Mix thoroughly. Aseptically and anaerobically .