tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 52

Handbook of Microbiological Media, Fourth Edition part 52 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Desulfobacca Medium 505 Yeast Extract Solution Composition per Yeast Preparation of Yeast Extract Solution Add yeast extract to dis-tilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. NaHCO3 Solution Composition per Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to . Mix thoroughly. Sparge with 20 CO2 80 H2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Vitamin Solution Calcium Folic Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Trace Elements Solution Composition per liter Nitrilotriacetic KAl SO4 Preparation of Trace Elements Solution Add nitrilotriacetic acid to of distilled deionized water. Dissolve by adjusting pH to with KOH. Add remaining components. Add distilled deionized water to . Mix thoroughly. Selenite Tungstate Solution Composition per liter Preparation of Selenite Tungstate Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except bicarbonate iron sulfate vitamins and organic substrates to distilled deionized water and bring volume to . Gently heat and bring to boiling. Boil for 1 min. Mix thoroughly. Cool to room temperature while sparging with 80 N2 20 CO2. Dispense into culture vessels under an atmosphere of 80 N2 20 CO2.

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