tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 194

Handbook of Microbiological Media, Fourth Edition part 194 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | YC Medium without Tryptophan 1925 Preparation of Medium Add components except methanol to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add filter-sterilized methanol. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of bacteria that can utilize methanol as a carbon source including Achromobacter methanolophila Methanomonas methylovora Methylobacterium species and Pseudomonas methanolica. YC Agar without Tryptophan Composition per liter Solution Solution Solution A Composition per Succinic NH4 L-Aspartic acid. L-Histidine. L-Proline. L-Serine . Calcium p-Aminobenzoic Folic pH at 25 C Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Filter sterilize. Warm to 50 -55 C. Solution B Composition per Agar Preparation of Solution B Add agar to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Preparation of Medium Aseptically mix of solution A and of solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the

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