tailieunhanh - báo cáo hóa học: " Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs | Journal of Neuroinflammation BioMed Central Research Open Access Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs Masato Hosokawa Andis Klegeris and Patrick L McGeer Address Kinsmen Laboratory of Neurological Research University of British Columbia 2255 Wesbrook Mall Vancouver BC V6T 1Z3 Canada Email Masato Hosokawa - mhosokaw@ Andis Klegeris - aklegeri@ Patrick LMcGeer - mcgeerpl@ Corresponding author Published 24 August 2004 Received 20 May 2004 Journal ofNeuroinflammation 2004 1 17 doi 1742-2094-1-17 Accepted 24 August 2004 This article is available from http content 1 1 17 2004 Hosokawa et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Oligodendrocytes neurons astrocytes microglia and endothelial cells are capable of synthesizing complement inhibitor proteins. Oligodendrocytes are vulnerable to complement attack which is particularly observed in multiple sclerosis. This vulnerability may be related to a deficiency in their ability to express complement regulatory proteins. Methods This study compared the expression level of complement inhibitor mRNAs by human oligodendrocytes astrocytes and microglia using semi-quantitative RT-PCR. Results Semi-quantitative RT-PCR analysis showed that C1 inhibitor C1-inh mRNA expression was dramatically lower in oligodendroglial cells compared with astrocytes and microglia. The mRNA expression level of membrane cofactor protein MCP by oligodendrocytes was also significantly lower than for other cell types. Conclusion The lower mRNA expression of C1-inh and MCP by oligodendrocytes could contribute to their vulnerability in .

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