tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 179

Handbook of Microbiological Media, Fourth Edition part 179 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thioglycolate Medium USP 1775 Thioglycolate Medium without Indicator Composition per liter Pancreatic digest of Papaic digest of soybean Sodium pH at 25 C Source This medium is available as a premixed powder from Oxoid Unipath. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Prepare freshly or boil and cool the medium just before use. Use For the growth of aerobic and anaerobic microorganisms in diagnostic bacteriology. Thioglycolate Medium without Indicator-135C Composition per liter Pancreatic digest of Papaic digest of soybean Sodium pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks filling them half full. For maintenance of cultures a small quantity of CaCO3 may be added to tubes before adding medium. Autoclave for 15 min at 13 psi pressure-118 C. Prepare freshly or boil and cool the medium just before use. Store prepared medium at 2 -8 C in the dark. Use For the isolation and cultivation of a wide variety of microorganisms particularly obligate anaerobes from clinical specimens and other materials. Thioglycolate Medium without Indicator with Hemin Thioglycolate Medium Supplemented Composition per liter Pancreatic digest of CaCO3 chips or Papaic digest of soybean Sodium Na2CO3 Vitamin K1 pH at 25 C Na2CO3 Solution