tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 178

Handbook of Microbiological Media, Fourth Edition part 178 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thiobacillus thioparus Medium 1765 pH at 25 C Preparation of Medium Add components except flowers of sulfur to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into flasks or bottles in volumes. Add of flowers of sulfur to each flask or bottle. Autoclave for 15 min at 15 psi pressure-121 C. Use For the isolation and cultivation of Thiobacillus thiooxidans. Thiobacillus thiooxidans Medium Composition per liter Sulfur pH at 25 C Preparation of Sulfur Add of powdered sulfur to a flask and sterilize by autoclaving for 15 min at 8 psi pressure-112 C. Preparation of Medium Add components except sulfur to dis-tilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with H2SO4. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically layer of sterile sulfur onto the surface of the medium. Use For the cultivation and maintenance of Thiobacillus species. Thiobacillus thioparus Agar Composition per liter Agar Na2S2O3-5H2O. NH4 pH at 25 C Preparation of Medium Add components except agar to dis-tilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Add agar. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 10 psi pressure-115 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of Thiobacillus thioparus. Thiobacillus thioparus Broth Composition per liter Na2S2O3-5H2O. NH4 pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to . Distribute .