tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 177

Handbook of Microbiological Media, Fourth Edition part 177 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thiobacillus caldus Medium 1755 Thiobacillus caldus Broth Composition per liter Solution Solution Solution Solution pH at 25 C Solution A Composition per NH4 Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with H2SO4. Autoclave for 15 min at 15 psi pressure-121 C. Solution B Composition per Ca NO3 MnSO4 Preparation of Solution B Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Solution C Composition per Preparation of Solution C Add glucose to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Solution D Composition per Preparation of Solution D Add Na2S4O6 to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Aseptically combine of sterile solution A with of sterile solution B of sterile solution C and of sterile solution D. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the heterotrophic cultivation and maintenance of Thiobacillus caldus. Thiobacillus caldus Broth Composition per liter Solution Solution Solution pH at 25 C Solution A Composition per NH4 Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Adjust pH to with H2SO4. Autoclave for 15 min at 15 psi pressure-121 C. Solution B Composition per Ca NO3 ZnSO4