tailieunhanh - báo cáo hóa học: " Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord | Kucharova et al. Journal of Neuroinflammation 2011 8 158 http content 8 1 158 JJOURNAL OF. NEUROINFLAMMATION RESEARCH Open Access Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord Karolina Kucharova1 Yunchao Chang1 2 Andrej Boor3 Voon Wee Yong4 and William B Stallcup1 Abstract Background Multiple sclerosis MS is a demyelinating disease in which blood-derived immune cells and activated microglia damage myelin in the central nervous system. While oligodendrocyte progenitor cells OPCs are essential for generating oligodendrocytes for myelin repair other cell types also participate in the damage and repair processes. The NG2 proteoglycan is expressed by OPCs pericytes and macrophages microglia. In this report we investigate the effects of NG2 on these cell types during spinal cord demyelination remyelination. Methods Demyelinated lesions were created by microinjecting 1 lysolecithin into the lumbar spinal cord. Following demyelination NG2 expression patterns in wild type mice were studied via immunostaining. Immunolabeling was also used in wild type and NG2 null mice to compare the extent of myelin damage the kinetics of myelin repair and the respective responses of OPCs pericytes and macrophages microglia. Cell proliferation was quantified by studies of BrdU incorporation and cytokine expression levels were evaluated using qRT-PCR. Results The initial volume of spinal cord demyelination in wild type mice is twice as large as in NG2 null mice. However over the ensuing 5 weeks there is a 6-fold improvement in myelination in wild type mice versus only a 2-fold improvement in NG2 null mice. NG2 ablation also results in reduced numbers of each of the three affected cell types. BrdU incorporation studies reveal that reduced cell proliferation is an important factor underlying NG2-dependent decreases in each of the three key cell populations. In addition NG2 ablation reduces macrophage microglial