tailieunhanh - báo cáo hóa học: " Microglia use multiple mechanisms to mediate interactions with vitronectin; non-essential roles for the highly-expressed avb3 and avb5 integrins"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Microglia use multiple mechanisms to mediate interactions with vitronectin; non-essential roles for the highly-expressed avb3 and avb5 integrins | Welser-Alves et al. Journal of Neuroinflammation 2011 8 157 http content 8 1 157 JJOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Microglia use multiple mechanisms to mediate interactions with vitronectin non-essential roles for the highly-expressed avp3 and avp5 integrins Jennifer V Welser-Alves Amin Boroujerdi Ulrich Tigges and Richard Milner Abstract Background As the primary resident immune cells microglia play a central role in regulating inflammatory processes in the CNS. The extracellular matrix ECM protein vitronectin promotes microglial activation switching microglia into an activated phenotype. We have shown previously that microglia express two vitronectin receptors avp3 and avp5 integrins. As these integrins have well-defined roles in activation and phagocytic processes in other cell types the purpose of the current study was to investigate the contribution of these two integrins in microglial activation. Methods Microglial cells were prepared from wild-type p3 integrin knockout KO p5 integrin KO or p3 p5 integrin DKO mice and their interactions and activation responses to vitronectin examined in a battery of assays including adhesion expression of activation markers MMP-9 expression and phagocytosis. Expression of other av integrins was examined by flow cytometry and immunoprecipitation. Results Surprisingly when cultured on vitronectin microglia from the different knockout strains showed no obvious defects in adhesion activation marker expression MMP-9 induction or phagocytosis of vitronectin-coated beads. To investigate the reason for this lack of effect we examined the expression of other av integrins. Flow cytometry showed that b3 b5 integrin DKO microglia expressed residual av integrin at the cell surface and immunoprecipitation confirmed this finding by revealing the presence of low levels of the avp1 and avp8 integrins. p1 integrin blockade had no impact on adhesion of b3 b5 integrin DKO microglia to vitronectin .