tailieunhanh - báo cáo hóa học: " Brain microvascular pericytes are immunoactive in culture: cytokine, chemokine, nitric oxide, and LRP-1 expression in response to lipopolysaccharide"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Brain microvascular pericytes are immunoactive in culture: cytokine, chemokine, nitric oxide, and LRP-1 expression in response to lipopolysaccharide | Kovac et al. Journal of Neuroinflammation 2011 8 139 http content 8 1 139 JIOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Brain microvascular pericytes are immunoactive in culture cytokine chemokine nitric oxide and LRP-1 expression in response to lipopolysaccharide Andrej Kovac2 4 Michelle A Erickson1 3 and William A Banks1 2 3 Abstract Background Brain microvascular pericytes are important constituents of the neurovascular unit. These cells are physically the closest cells to the microvascular endothelial cells in brain capillaries. They significantly contribute to the induction and maintenance of the barrier functions of the blood-brain barrier. However very little is known about their immune activities or their roles in neuroinflammation. Here we focused on the immunological profile of brain pericytes in culture in the quiescent and immune-challenged state by studying their production of immune mediators such as nitric oxide NO cytokines and chemokines. We also examined the effects of immune challenge on pericyte expression of low density lipoprotein receptor-related protein-1 LRP-1 a protein involved in the processing of amyloid precursor protein and the brain-to-blood efflux of amyloid-p peptide. Methods Supernatants were collected from primary cultures of mouse brain pericytes. Release of nitric oxide NO was measured by the Griess reaction and the level of S-nitrosylation of pericyte proteins measured with a modified biotin-switch method. Specific mitogen-activated protein kinase MAPK pathway inhibitors were used to determine involvement of these pathways on NO production. Cytokines and chemokines were analyzed by multianalyte technology. The expression of both subunits of LRP-1 was analyzed by western blot. Results Lipopolysaccharide LPS induced release of NO by pericytes in a dose-dependent manner that was mediated through MAPK pathways. Nitrative stress resulted in S-nitrosylation of cellular proteins. Eighteen of .