tailieunhanh - báo cáo hóa học: " Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairment"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairment | VanGuilder et al. Journal of Neuroinflammation 2011 8 138 http content 8 1 138 JJOURNAL OF. NEUROINFLAMMATION RESEARCH Open Access Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairment 1 1 12 2 2 Heather D VanGuilder Georgina V Bixler Robert M Brucklacher Julie A Farley Han Yan Junie P Warrington William E Sonntag2 and Willard M Freeman1 Abstract Background Age-related cognitive dysfunction including impairment of hippocampus-dependent spatial learning and memory affects approximately half of the aged population. Induction of a variety of neuroinflammatory measures has been reported with brain aging but the relationship between neuroinflammation and cognitive decline with non-neurodegenerative normative aging remains largely unexplored. This study sought to comprehensively investigate expression of the MHC II immune response pathway and glial activation in the hippocampus in the context of both aging and age-related cognitive decline. Methods Three independent cohorts of adult 12-13 months and aged 26-28 months F344xBN rats were behaviorally characterized by Morris water maze testing. Expression of MHC II pathway-associated genes identified by transcriptomic analysis as upregulated with advanced aging was quantified by qPCR in synaptosomal fractions derived from whole hippocampus and in hippocampal subregion dissections CA1 CA3 and DG . Activation of astrocytes and microglia was assessed by GFAP and Iba1 protein expression and by immunohistochemical visualization of GFAP and both CD74 Ox6 and Iba1. Results We report a marked age-related induction of neuroinflammatory signaling transcripts . MHC II components toll-like receptors complement and downstream signaling factors throughout the hippocampus in all aged rats regardless of cognitive status. Astrocyte and microglial activation was evident in CA1 CA3 and DG of intact and impaired aged rat .