tailieunhanh - báo cáo hóa học: " Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells | Dao et al. Journal of Neuroinflammation 2011 8 133 http content 8 1 133 JJOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Comparing the immunosuppressive potency of naive marrow stromal cells and Notch-transfected marrow stromal cells Mo A Dao1 Ciara C Tate1 Irina Aizman1 Michael McGrogan2 and Casey C Case1 Abstract Background SB623 cells are expanded from marrow stromal cells MSCs transfected with a Notch intracellular domain NICD -expressing plasmid. In stroke-induced animals these cells reduce infarct size and promote functional recovery. SB623 cells resemble the parental MSCs with respect to morphology and cell surface markers despite having been in extended culture. MSCs are known to have immunosuppressive properties whether longterm culture of MSCs impact their immunomodulatory activity has not been addressed. Methods To assess the possible senescent properties of SB623 cells we performed cell cycle related assays and beta-galactosidase staining. To assess the immunomodulatory activity of these expanded NICD-transfected MSCs we performed co-cultures of SB623 cells or MSCs with either enriched human T cells or monocytes and assessed cytokine production by flow cytometry. In addition we monitored the immunosuppressive activity of SB623 cells in both allogenic and xenogenic mixed lymphocyte reaction MLR . Results Compared to MSCs we showed that a small number of senescent-like cells appear in each lot of SB623 cells. Nevertheless we demonstrated that these cells suppress human T cell proliferation in both the allogeneic and xenogeneic mixed lymphocyte reaction MLR in a manner comparable to MSCs. IL-10 producing T cells were generated and monocyte-dendritic cell differentiation was dampened by co-culture with SB623 cells. Compared to the parental MSCs SB623 cells appear to exert a greater inhibitory impact on the maturation of dendritic cells as demonstrated by a greater reduction in the surface expression of the co-stimulatory .