tailieunhanh - báo cáo hóa học: " Sinomenine inhibits microglial activation by Ab and confers neuroprotection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Sinomenine inhibits microglial activation by Ab and confers neuroprotection | Shukla and Sharma Journal of Neuroinflammation 2011 8 117 http content 8 1 117 JJOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Sinomenine inhibits microglial activation by Ab and confers neuroprotection Shilpa Mishra Shukla and Shiv K Sharma Abstract Background Neuroinflammation is an important contributor to the development of neurodegenerative diseases including Alzheimer s disease. Thus there is a keen interest in identifying compounds especially from herbal sources that can inhibit neuroinflammation. Amyloid-p Ab is a major component of the amyloid plaques present in the brains of Alzheimer s disease patients. Here we examined whether sinomenine present in a Chinese medicinal plant prevents oligomeric Ab-induced microglial activation and confers protection against neurotoxicity. Methods Oligomeric amyloid-p was prepared from Ab 1-42 . Intracellular reactive oxygen species production was determined using the dye 2 7 -dichlorodihydrofluorescin diacetate. Nitric oxide level was assessed using the Griess reagent. Flow cytometry was used to examine the levels of inflammatory molecules. BV2-conditioned medium was used to treat hippocampal cell line HT22 and primary hippocampal cells in indirect toxicity experiments. Toxicity was assessed using MTT reduction and TUNEL assays. Results We found that sinomenine prevents the oligomeric Ap-induced increase in levels of reactive oxygen species and nitric oxide in BV2 microglial cells. In addition sinomenine reduces levels of Ap-induced inflammatory molecules. Furthermore sinomenine protects hippocampal HT22 cells as well as primary hippocampal cells from indirect toxicity mediated by Ap-treated microglial cells but has no effect on Ap-induced direct toxicity to HT22 cells. Finally we found that conditioned medium from Ap-treated BV2 cells contains increased levels of nitric oxide and inflammatory molecules but the levels of these molecules are reduced by sinomenine. Conclusions Sinomenine .