tailieunhanh - báo cáo hóa học: " A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells | Furr et al. Journal of Neuroinflammation 2011 8 99 http content 8 1 99 JJOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells Samantha R Furr Vinita S Chauhan Megan J Moerdyk-Schauwecker and Ian Marriott Abstract Background The rapid onset of potentially lethal neuroinflammation is a defining feature of viral encephalitis. Microglia and astrocytes are likely to play a significant role in viral encephalitis pathophysiology as they are ideally positioned to respond to invading central nervous system CNS pathogens by producing key inflammatory mediators. Recently DNA-dependent activator of IFN regulatory factor DAI has been reported to function as an intracellular sensor for DNA viruses. To date the expression and functional role of DAI in the inflammatory responses of resident CNS cells to neurotropic DNA viruses has not been reported. Methods Expression of DAI and its downstream effector molecules was determined in C57BL 6-derived microglia and astrocytes either at rest or following exposure to herpes simplex virus type 1 HSV-1 and or murine gammaherpesvirus-68 MHV-68 by immunoblot analysis. In addition such expression was studied in ex vivo microglia macrophages and astrocytes from uninfected animals or mice infected with HSV-1. Inflammatory cytokine production by glial cultures following transfection with a DAI specific ligand B-DNA or following HSV-1 challenge in the absence or presence of siRNA directed against DAI was assessed by specific capture ELISA. The production of soluble neurotoxic mediators by HSV-1 infected glia following DAI knockdown was assessed by analysis of the susceptibility of neuron-like cells to conditioned glial media. Results We show that isolated microglia and astrocytes constitutively express DAI and its effector molecules and show that such expression is upregulated following DNA .