tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 162

Handbook of Microbiological Media, Fourth Edition part 162 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Spirochaeta litoralis Medium 1605 Glucose Salts Solution Composition per liter Glucose Sulfide Preparation of Glucose Salts Solution Add components except glucose solution and sulfide solution to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Aseptically add sterile glucose solution and sulfide solution. Mix thoroughly. Sulfide Solution Composition per Preparation of Sulfide Solution Add Na2S-9H2O to distilled de-ionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Glucose Solution Composition per Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to . Mix thoroughly. Filter sterilize. Yeast Extract Peptone Solution Composition per Yeast Preparation of Yeast Extract Peptone Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Preparation of Medium Aseptically combine of yeast extract peptone solution with of glucose salts solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the isolation and cultivation of Spirochaeta halophila. Spirochaeta isovalerica Medium Composition per liter Pancreatic digest of Yeast g Tris-HCl buffer solution pH . pH at 25 C Preparation of Medium Prepare and dispense medium under 100 N2. Combine components. Mix thoroughly. Sparge with 100 N2. Adjust pH to while continuing to sparge with 100 N2. Anaerobically distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and .

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