tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 153

Handbook of Microbiological Media, Fourth Edition part 153 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Rogosa Broth Modified 1515 Yeast Iron III Bromthymol Ampicillin pH at 25 C Source This medium without ampicillin solution and ethanol is available as a premixed powder from HiMedia. Ampicillin Solution Composition per Preparation of Ampicillin Solution Add ampicillin to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except ampicillin solution and ethanol to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add of sterile ampicillin solution and of ethanol. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the isolation cultivation and differentiation of Aeromonas species and Plesiomonas species from water samples using the membrane filter method. For the differential and selective isolation of Aer-omonas hydrophila species from water samples RM Medium Composition per liter Yeast Solution Solution Solution Solution pH at 25 C Solution 1 Composition per Preparation of Solution 1 Add glucose to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Solution 2 Composition per Preparation of Solution 2 Add agar to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Solution 3 Composition per Yeast Preparation of Solution 3 Add yeast extract to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. .