tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 151

Handbook of Microbiological Media, Fourth Edition part 151 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Rhodobium gokurnum Medium 1495 slowly add of sterile 2M H2SO4. This partially neutralizes the solution. The solution should turn yellow. H2S gas will be liberated neutralization and distribution of the solution should be done as rapidly as possible under adequate ventilation. Preparation of Medium To the of sterile solution 1 in screw-capped bottles add combined solutions 2 and 3 immediately after filtration and fill bottles to capacity. Mix thoroughly. Aseptically remove of the medium from the bottles and replace it with of neutralized solution 4. Let stand for 24 hr. The medium should form a fine white precipitate before using. To inoculate remove of the completed medium from the bottles and replace it with of inoculum. Use For the cultivation and maintenance of Rhodobacter veldkampii. Rhodobium gokurnum Medium DSMZ Medium 1129 Composition per liter NH4Cl . Sorbitol Yeast extract Sodium pyruvate Ferric citrate Trace elements solution pH at 25 C Ferric Citrate Solution Composition per Ferric Preparation of Ferric Citrate Solution Add ferric citrate to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Trace Elements Solution SL-7 Composition per liter HCl 25 . Preparation of Trace Elements Solution SL-7 Add components to distilled deionized water and bring volume to . Mix thoroughly. Sorbitol Solution Composition per Sorbitol. Preparation of Sorbitol Solution Add sorbitol to distilled deion-ized water and bring volume to . Mix thoroughly. Filter sterilize. Sodium Pyruvate Solution Composition per Sodium pyruvate . Preparation of Sodium Pyruvate Solution Add sodium pyruvate to .