tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 148

Handbook of Microbiological Media, Fourth Edition part 148 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Pyrococcus furiosus Medium 1465 Pyrobaculum Medium Composition per liter NH4 Yeast Preparation of Medium Add components except peptone yeast extract and Na2S-9H2O to distilled deionized water and bring volume to . Mix thoroughly. Bring pH to using NaOH. Sparge with 100 N2 for 30 min. Add peptone yeast extract and Na2S-9H2O. Bring pH back to using 10A H2SO4. Anaerobically distribute into sterile tubes or flasks under 100 N2. Do not autoclave medium. If not used immediately heat the medium to 90 C for 60 min on each of 3 consecutive days. Use For the cultivation and maintenance of Pyrobaculum islandicum. Pyrobaculum Medium Composition per liter Sulfur NH4 Peptone. Yeast Preparation of Medium Add components except peptone yeast extract and Na2S-9H2O to distilled deionized water and bring volume to . Mix thoroughly. Bring pH to using NaOH. Sparge with 100 N2 for 30 min. Add peptone yeast extract and Na2S-9H2O. Bring pH back to using 10A H2SO4. Anaerobically distribute into sterile tubes or flasks under 100 N2. Do not autoclave medium. If not used immediately heat the medium to 90 C for 60 min on each of three consecutive days. Use For the cultivation and maintenance of Pyrobaculum organotro-phum. Pyrococcus endeavori Medium ES4 Composition per Solution Solution Solution Solution A Composition per liter Preparation of Solution A Add .