tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 147

Handbook of Microbiological Media, Fourth Edition part 147 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | PYEM Medium 1455 Wolfe s Mineral Solution Composition per liter Nitrilotriacetic AlK SO4 Preparation of Wolfe s Mineral Solution Add nitrilotriacetic acid to approximately of water and adjust to pH with KOH to dissolve the compound. Bring volume to with remaining water and add remaining components one at a time. Preparation of Medium Add components except fructose solution and NaHCO3 solution to distilled deionized water and bring volume to . Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add of sterile fructose solution and of sterile NaHCO3 solution. Mix thoroughly. Adjust pH to . Aseptically distribute into sterile tubes or flasks. Fill containers to capacity. Use For the cultivation of Rhodoferax fermentans and Thiocapsa halophila. PYE Medium Composition per liter Yeast Sodium NH4 Trace elements solution Trace Elements Solution SL-6 Composition per liter ZnSO4-7H2O. Na2MoO4-2H2O. Preparation of Trace Elements Solution SL-6 Add components to distilled deionized water and bring volume to . Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Heliobacterium modestocaldum. PYEA Agar Composition per liter Yeast NaCl. pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave

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