tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 144

Handbook of Microbiological Media, Fourth Edition part 144 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | PRAS-PYG with Tween 80 1425 Beef heart solids from Mycoploasma supplement Penicillin pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Mycoplasma Supplement Solution Composition per Horse Yeast extract fresh autolysate . Thallium mg Preparation of Mycoplasma Supplement Solution Combine components. Mix thoroughly. Filter sterilize. Penicllin Solution Composition per 000U Preparation of Penicllin Solution Add penicillin to distilled de-ionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Mycoplasma supplement solution and penicllin solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile Mycoplasma supplement solution and penicllin solution. Mix thoroughly. Use For the selective cultivation of Mycoplasma species. PPNG Selective Medium Penicillinase-Producing Neisseria gonorrhoeae Medium Composition per plate Quadrant Quadrant Quadrant I Composition per Martin Lewis Quadrant II Composition per Martin Lewis agar Use For the differentiation and presumptive identification of penicillinase-producing strains of Neisseria gonorrhoeae. The PPNG selective medium is a two-sectored plate each containing a different medium. See Martin-Lewis agars for additional information. PPT Agar 1M Composition per liter Proteose peptone No. Pancreatic digest of Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri .