tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 143

Handbook of Microbiological Media, Fourth Edition part 143 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Potato Dextrose Yeast Agar 1415 Preparation of Potato Infusion Peel and dice potatoes. Add of distilled deionized water. Gently heat and bring to boiling. Continue boiling for 60 min. Filter through cheesecloth. Bring volume to with distilled deionized filtrate. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of yeasts and molds. Potato Dextrose Broth Composition per liter Potatoes infusion pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Potatoes Infusion From Composition per Preparation of Potatoes Infusion From Peel and dice potatoes. Add of distilled deionized water. Gently heat and bring to boiling. Continue boiling for 30 min. Filter through cheesecloth. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of a wide variety of yeasts and molds. Potato Dextrose Broth with Yeast Extract Composition per liter Potato peeled and Yeast pH at 25 C Preparation of Medium Peel and cut potatoes. Add potatoes to of water. Boil potatoes for 20 min. Filter through cheesecloth. Add glucose and yeast extract to filtrate. Bring volume to with distilled deionized water . Mix thoroughly. Adjust pH to . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of a variety of fungi. Potato Dextrose L-IsoleucineAgar ATCC Medium 2205 Composition per liter Potatoes infusion L-Isoleucine