tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 142

Handbook of Microbiological Media, Fourth Edition part 142 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | PMYA II Medium 1405 Antibiotic Inhibitor Composition per 000U 000U Preparation of Antibiotic Inhibitor Add components to dis-tilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except antibiotic inhibitor to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the selective isolation and cultivation of Bacillus anthracis. Pluton Medium Composition per Malt Yeast Trypticase . Potassium phosphate buffer 1M solution pH . L-Cysteine-HCl pH at 25 C L-Cysteine-HCl Solution Composition per Preparation of L-Cysteine-HCl Solution Add L-cysteine-HCl to distilled deionized water and bring volume to . Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except L-cysteine-HCl solution to distilled deionized water and bring volume to . Mix thoroughly. Distribute volumes into tubes. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add of sterile L-cysteine-HCl solution to each tube. Mix thoroughly. Use For the cultivation of Melissococcus pluton. PM Indicator Agar Composition per liter Beef Pancreatic digest of Sorbitan monooleate Papaic digest of soybean Bromcresol pH at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix .