tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 136

Handbook of Microbiological Media, Fourth Edition part 136 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Park and Sanders Enrichment Hi Veg Broth Base with Horse Blood and Selective Antibiotics 1345 Folic a-Lipoic Preparation of Supplement A Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Supplement B Composition per Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Distribute while stirring into screw-capped tubes in volumes. Store at -20 C. Cycloheximide. Thaw as needed. Solution B Composition per TEM-4T Hachmeister Pittsburgh . Ethanol Preparation of Solution B Add TEM-4T and stigmasterol to of hot ethanol. Mix thoroughly. Store at 4 C. Solution C Composition per Proteose Pancreatic digest of Ribonucleic Preparation of Solution C Add components to distilled deionized water and bring volume to . Mix thoroughly. Preparation of Medium Combine of solution C of solution A and of solution B. Mix thoroughly. Bring volume to with distilled deionized water. Adjust pH to with N NaOH. Autoclave for 20 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically distribute into sterile tubes or flasks. Use For the cultivation ofParamecium species to be used as host cells by bacterial symbionts. Park and Sanders Enrichment Broth Composition per Preparation of Supplement B Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium To of cooled sterile basal medium aseptically add of sterile supplement A. Mix thoroughly. Aseptically distribute into flasks in volumes. Inoculate medium with food samples. .