tailieunhanh - Báo cáo y học: " A comparative analysis of HIV drug resistance interpretation based on short reverse transcriptase sequences versus full sequences"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: A comparative analysis of HIV drug resistance interpretation based on short reverse transcriptase sequences versus full sequences. | Steegen et al. AIDS Research and Therapy 2010 7 38 http content 7 1 38 AIDS RESEARCH AND THERAPY RESEARCH Open Access A comparative analysis of HIV drug resistance interpretation based on short reverse transcriptase sequences versus full sequences IVino ItomoAi liWollo R oevo2 Ribo r_ io o ld o l 3 Ruet lA lntcurc4 l eud u nn ol ur Romtt3 d .i olo I A olllc2 Kim Steegen Mliciielle Bronze Elke Van Cldeiieiiuioecr. Dall Winters Koen Van del DUiynt Carole L Wallis Wendy Stevens5 Tobias F Rlnke de Wil6 Lieven J Sluyver1 the ART-A consortium7 8 9 10 11 12 13 Abstract Background As second-line antiretroviral treatment ART becomes more accessible in resource-limited settings RLS the need for more affordable monitoring tools such as point-of-care viral load assays and simplified genotypic HIV drug resistance HIVDR tests increases substantially. The prohibitive expenses of genotypic HIVDR assays could partly be addressed by focusing on a smaller region of the HIV reverse transcriptase gene RT that encompasses the majority of HIVDR mutations for people on ART in RLS. In this study an in silico analysis of 125 329 RT sequences was performed to investigate the effect of submitting short RT sequences codon 41 to 238 to the commonly used virco TYPE and Stanford genotype interpretation tools. Results Pair-wise comparisons between full-length and short RT sequences were performed. Additionally a noninferiority approach with a concordance limit of 95 and two-sided 95 confidence intervals was used to demonstrate concordance between HIVDR calls based on full-length and short RT sequences. The results of this analysis showed that HIVDR interpretations based on full-length versus short RT sequences using the Stanford algorithms had concordance significantly above 95 . When using the virco TYPE algorithm similar concordance was demonstrated 95 but some differences were observed for d4T AZT and TDF where predictions were affected in more than 5 of the sequences. .