tailieunhanh - Báo cáo y học: "The identification of unique serum proteins of HIV-1 latently infected long-term non-progressor patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: The identification of unique serum proteins of HIV-1 latently infected long-term non-progressor patients. | Van Duyne et al. AIDS Research and Therapy 2010 7 21 http content 7 1 21 AIDS RESEARCH AND THERAPY RESEARCH Open Access The identification of unique serum proteins of HIV-1 latently infected long-term non-progressor patients Rachel Van Duyne1 2 Irene Guendel2 Kylene Kehn-Hall2 Rebecca Easley2 Zachary Klase3 Chenglong Liu4 Mary Young4 and Fatah Kashanchi 2 5 Abstract Background The search for disease biomarkers within human peripheral fluids has become a favorable approach to preventative therapeutics throughout the past few years. The comparison of normal versus disease states can identify an overexpression or a suppression of critical proteins where illness has directly altered a patient s cellular homeostasis. In particular the analysis of HIV-1 infected serum is an attractive medium with which to identify altered protein expression due to the ease and non-invasive methods of collecting samples as well as the corresponding insight into the in vivo interaction of the virus with infected cells tissue. The utilization of proteomic techniques to globally identify differentially expressed serum proteins in response to HIV-1 infection is a significant undertaking that is complicated due to the innate protein profile of human serum. Results Here the depletion of 12 of the most abundant serum proteins followed by two-dimensional gel electrophoresis coupled with identification of these proteins using matrix-assisted laser desorption ionization time-of-flight MALDI-TOF mass spectrometry has allowed for the identification of differentially expressed low abundant serum proteins. We have analyzed and compared serum samples from HIV-1 infected subjects who are being treated using highly active antiretroviral therapy HAART to those who are latently infected but have not progressed to AIDS despite the absence of treatment . long term non-progressors LTNPs . Here we have identified unique serum proteins that are differentially expressed in LTNP HIV-1 .

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