tailieunhanh - Handbook of Microbiological Media, Fourth Edition part 119

Handbook of Microbiological Media, Fourth Edition part 119 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Mineral Medium with Dichloromethane 1175 Folic Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Cyanuric Acid Solution Composition per Cyanuric Preparation of Cyanuric Acid Solution Add cyanuric acid to distilled deionized water. Mix thoroughly. Adjust pH to . Filter sterilize. Glycerol Solution Composition per Preparation of Glycerol Solution Add glycerol to distilled de-ionized water. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except cyanuric acid solution glycerol solution and vitamin solution to distilled deionized water. Adjust pH to . Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add cyanuric acid solution glycerol solution and vitamin solution. Mix thoroughly. Aseptically distribute to sterile tubes or flasks. Use For the cultivation of Gordonia rubripertincta Rhodococcus rubropertinctus and other cyanuric acid-utilizing bacteria. Mineral Medium with Dichlorobenzoate Composition per liter NH4 Hutner s mineral 2 4-Dichlorobenzoate pH at 25 C Hutner s Mineral Base Composition per liter Nitrilotriacetic NH4 Metals 44 . Preparation of Hutner s Mineral Base Add nitrilotriacetic acid to of distilled deionized water. Dissolve by adjusting pH to with KOH. Add remaining components. Readjust pH to with H2SO4 or KOH. Add distilled deionized water to . Metals 44 Composition per Co NO3 Preparation of Metals 44 Add a few drops of H2SO4 to dis-tilled deionized water to inhibit precipitate formation. Add .